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The two protein bands from forty kDa to 30 kDa have been absent just after 24 h incubation at 45 °C. For the rest of the bands, numerous concentrations of all a few proteases retained protein bands equivalent to their unique molar masses (Determine 6a,b). The presence of bands at very low molecular excess weight in fish roe in advance of hydrolysis was sudden. Within the report by Ghelichi et al. [eight], minimal peaks of a relatively very low molecular weight selection (three–14 kDa) had been noticed in defatted carp roe homogenate, possibly as a consequence of an autolytic procedure transpiring in fish roe and/or maybe the defatting course of action leading to the formation of very low-molecular-weight peptides.
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